Cloning Vectors – Section 1

21. EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to

A. 6-7 kb
B. 15-25 kb
C. 40-44 kb
D. 1-2 kb

Correct Answer: B. 15-25 kb
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22. Size of the DNA that can be packaged into a λ phage is

A. 50 kb
B. 35-53 kb
C. 40-50 kb
D. any size

Correct Answer: B. 35-53 kb
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23. Charon 34 and Charon 35 are the examples of

A. plasmid vector
B. cosmid vector
C. phage vector
D. phagemid vector

Correct Answer: C. phage vector
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24. Cosmid vectors are used for

A. cloning small fragments of DNA
B. cloning large fragments of DNA
C. cloning prokaryotic DNA only
D. cloning eukaryotic DNA only

Correct Answer: B. cloning large fragments of DNA
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25. Plasmids which are maintained as a limited number of copies per cell are known as

A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. all of these

Correct Answer: A. stringent plasmids
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