Cloning Vectors – Section 1

16. Cos site of the cosmids

A. consists of 12 bases
B. helps whole genome in circularization and ligation
C. both (a) and (b)
D. contains cleavage site

Correct Answer: C. both (a) and (b)

17. M 13 is an example of

A. filamentous phage
B. single stranded DNA vector
C. both (a) and (b)
D. plasmid

Correct Answer: C. both (a) and (b)

18. Phagemid vectors are

A. combination of plasmid and phage λ
B. combination of phages and cosmid
C. phages carrying properties of plasmids
D. all of the above

Correct Answer: A. combination of plasmid and phage λ

19. Single-stranded vectors are useful

A. for sequencing of cloned DNA
B. for oligonucleotide-directed mutagenesis
C. for probe preparation
D. all of the above

Correct Answer: D. all of the above

20. Inserted DNA in λ gt 11 can be expressed as

A. β-galactosidase fused protein
B. free protein in the cytoplasm
C. free protein that is secreted out
D. all of the above

Correct Answer: A. β-galactosidase fused protein

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