Cloning Vectors – Section 1

11. Plasmid incompatibility is

A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. both (a) and (b)
D. none of the above

Correct Answer: B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.

12. P1 cloning vector allows cloning of DNA of the length of

A. 100 kbp
B. 50 kbp
C. 20 kbp
D. 10 kbp

Correct Answer: A. 100 kbp

13. Charon 34 and Charon 35 can clone DNA upto

A. 1-2 kb
B. 6-7 kb
C. 9-20 kb
D. 30-35 kb

Correct Answer: C. 9-20 kb

14. Charon vectors are different from EMBL vectors because

A. they have more extensive range of restriction targets within their polylinkers.
B. physical separation of lambda arm from central fragment is required
C. both (a) and (b)
D. physical separation of lambda arm from central fragment is not required

Correct Answer: C. both (a) and (b)

15. P1 cloning vector is the example of

A. plasmid
B. cosmid
C. bacteriophage
D. phagemid

Correct Answer: C. bacteriophage

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