Cloning Vectors – Section 1

6. λ ZAP vector is an example of

A. phage
B. phagemid
C. cosmid
D. plasmid

Correct Answer: B. phagemid

7. λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

A. 6-7 kb
B. 1-2 kb
C. 40-44 kb
D. 20-23 kb

Correct Answer: A. 6-7 kb

8. Select the wrong statement about plasmids?

A. It is extrachromosomal
B. It is double-stranded
C. Its replication depends upon host cell
D. It is closed and circular DNA

Correct Answer: C. Its replication depends upon host cell

9. Stuffer is

A. the right arm of the vector DNA
B. the left arm of the vector DNA
C. central fragment of the vector DNA
D. none of the above

Correct Answer: C. central fragment of the vector DNA

10. Conjugative plasmids

A. exhibit antibiotic resistance
B. do not exhibit antibiotic resistance
C. carry transfer genes called the tra genes
D. do not carry transfer genes

Correct Answer: C. carry transfer genes called the tra genes

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