Cloning Vectors – Section 1

1. Maximum size of foreign DNA that can be inserted into a replacement vector is

A. 25-30 kb
B. 18-20 kb
C. 20-25 kb
D. 40-50 kb

Correct Answer: C. 20-25 kb

2. Which of the following is not true about phagemid?

A. Contain functional origin of replication of the plasmid and λ phage
B. May be propagated as a plasmid or as phage in appropriate strain
C. Contain λ att site
D. Can only be propagated as phage

Correct Answer: D. Can only be propagated as phage

3. pBR 322 has/have which of the following selection marker(s)?

A. Ampr
B. Tetr
C. Both (a) and (b)
D. Kanr

Correct Answer: C. Both (a) and (b)

4. A plasmid can be considered as a suitable cloning vector if

A. it can be readily isolated from the cells
B. it possesses a single restriction site for one or more restriction enzymes
C. insertion of foreign DNA does not alter its replication properties
D. All of the above

Correct Answer: D. All of the above

5. Difference between λ gt 10 and λ gt 11 vectors is that

A. λ gt 11 is an expression vector
B. λ gt 10 is an expression vector
C. λ gt 10 is a replacement vector
D. λ gt 11 is a replacement vector

Correct Answer: A. λ gt 11 is an expression vector

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