Cloning Vectors – Section 1

26. Cryptic plasmids

A. do not exhibit any phenotypic trait
B. exhibit many phenotypic traits
C. exhibit one phenotypic traits
D. exhibit antibiotic resistance

Correct Answer: A. do not exhibit any phenotypic trait

27. Phagemid consist of

A. plasmid vector carrying λ phage’s cos site
B. plasmid vector carrying λ attachment (λ att) site
C. plasmid vector carrying origin of replication of λ phage only
D. plasmid vector carrying origin of replication of plasmid only

Correct Answer: B. plasmid vector carrying λ attachment (λ att) site

28. Maximum size of foreign DNA that can be inserted into an insertion vector is

A. 35 kb
B. 18 kb
C. 50 kb
D. 27 kb

Correct Answer: B. 18 kb

29. Plasmids which are maintained as multiple copy number per cell are known as

A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. none of these

Correct Answer: B. relaxed plasmids

30. Cosmid vectors are

A. plasmids that contain fragment of λ DNA including the cos site
B. phages that lack cos site
C. plasmids that have no selection marker
D. cryptic plasmids

Correct Answer: A. plasmids that contain fragment of λ DNA including the cos site

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