Vectors Uses for Animal Cell Culture – Section 1


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6. Which of the following histone is not present in the SV40 virus?

A. H1
B. H2A
C. H3
D. H4

Correct Answer: A. H1
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7. Autonomously replicating sequence is not present in

A. YIp
B. YRp
C. YCp
D. YAC

Correct Answer: A. YIp
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8. In the case of Baculoviruses, the foreign gene

A. is inserted downstream of the polyhedrin promoter
B. is inserted downstream of the viral origin of replication
C. is inserted between the polyhedrin promoter and downstream sequences
D. is inserted randomly in the viral genome

Correct Answer: C. is inserted between the polyhedrin promoter and downstream sequences
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9. α-aminoadipate inhibits the growth if one of the following markers is present in the yeast. That is

A. His 3
B. Lys 2
C. Ura 3
D. Trp 1

Correct Answer: B. Lys 2
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10. Yeast episomal plasmids (YEp) were constructed using

A. 2μm yeast plasmid, fragments of yeast nuclear DNA and E Coli vector pMB 9
B. 2μm yeast plasmid, fragments of yeast nuclear DNA and E Coli vector pBR 322
C. 12μm yeast plasmid and E Coli vector pMB 9
D. 12μm yeast plasmid and E Coli vector pBR 322

Correct Answer: A. 2μm yeast plasmid, fragments of yeast nuclear DNA and E Coli vector pMB 9
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