Vectors Uses for Animal Cell Culture – Section 1

16. Cloning genes in the yeast offer which of following advantage(s)?

A. Overproduction of proteins of commercial value
B. Ability to glycosylate proteins during secretion
C. Ability to clone large piece of DNA
D. All of the above

Correct Answer: D. All of the above

17. Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

A. Allows transformation by using crossing over
B. Has an origin of replication and rep genes
C. Carries autonomously replicating sequence
D. Carries centromere and telomere sequences

Correct Answer: B. Has an origin of replication and rep genes

18. Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

A. Contains centromeric sequences
B. Segregates during meiosis and mitosis
C. Functions as true chromosome
D. All of the above

Correct Answer: D. All of the above

19. Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

A. Vaccinia virus
B. Baculovirus
C. Papillomavirus
D. Retrovirus

Correct Answer: B. Baculovirus

20. Polyhedrin protein is

A. nonessential for baculovirus replication
B. major component of the protein matrix in which viruses are embedded
C. both (a) and (b)
D. essential for baculovirus replication

Correct Answer: C. both (a) and (b)

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