Vectors Uses for Animal Cell Culture – Section 1

1. Yeast Replicating plasmids (YRp) carry

A. centromere
B. telomere
C. autonomously replicating sequence
D. both (a) and (c)

Correct Answer: C. autonomously replicating sequence

2. Which of the following viruses are used as vectors for transferring genes to animal cells?

A. SV 40 virus
B. Bovine papillomavirus (BVP)
C. Retroviruses
D. All of these

Correct Answer: D. All of these

3. Recombinant adenovirus can be created by deleting E1A/E 1B genes that are

A. transcriptional regulators and are essential for viral replication
B. not necessary for viral replication
C. coding for virus coat protein
D. important for packaging of viral DNA into the viral head

Correct Answer: A. transcriptional regulators and are essential for viral replication

4. Retroviruses have an advantage for being used as a vector for animal cells because

A. they cover a wide host range
B. infection does not lead to cell death, infected cells produce virus over an indefinite period
C. viral gene expression is driven by strong promoters
D. all of the above

Correct Answer: D. all of the above

5. Using bovine papillomavirus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

A. episomally only
B. integrated only
C. either episomally or integrated at high copy number
D. either episomally or integrated at low copy number

Correct Answer: C. either episomally or integrated at high copy number

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