Vectors Uses for Animal Cell Culture – Section 1

11. Which of the following is/are commonly used promoters of S. cerevisiae?

A. Galactokinase (GAL 1)
B. Alcohol dehydrogenase II (ADH 2)
C. Phosphoglycerate kinase (PGK)
D. All of the above

Correct Answer: D. All of the above

12. The maximum amount of foreign DNA introduced into SV 40 viruses is

A. 10 kb
B. 5 kb
C. 2.5 kb
D. 1 kb

Correct Answer: C. 2.5 kb

13. Recombinant SV40 viruses introduce the foreign DNA into cells

A. with the DNA mediated transfection
B. without the DNA mediated transfection
C. with the RNA mediated transfection
D. without the RNA mediated transfection

Correct Answer: B. without the DNA mediated transfection

14. Which one of the following is the most stable vector?

A. YEp
B. YRp
C. YAC
D. YIp

Correct Answer: C. YAC

15. SV 40 vectors, in which early region replacements are carried out, require

A. COS cell line
B. SV 40 helper virus
C. pBR 322 vector
D. none of these

Correct Answer: A. COS cell line

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