Gene Transfer in Plants – Section 1

1. Virulence trait of Agrobacterium tumefaciens is borne on

A. chromosomal DNA
B. tumor inducing plasmid DNA
C. both chromosomal and plasmid DNA
D. cryptic plasmid DNA

Correct Answer: B. tumor inducing plasmid DNA

2. The size of the virulent plasmid of Agrobacterium tumefaciens is

A. 40-80 kb
B. 80-120 kb
C. 140-235 kb
D. >235 kb

Correct Answer: C. 140-235 kb

3. Which of the following is not true about the helper plasmids?

A. These can replicate in Agrobacterium
B. These help in the mediating conjugation of intermediate vectors
C. These can’t replicate in Agrobacterium
D. All of the above

Correct Answer: A. These can replicate in Agrobacterium

4. Which technique is used to introduce genes into dicots?

A. electroporation
B. particle acceleration
C. microinjection
D. Ti plasmid infection

Correct Answer: D. Ti plasmid infection

5. Direct DNA uptake by protoplasts can be stimulated by

A. polyethylene glycol (PEG)
B. decanal
C. luciferin
D. all of these

Correct Answer: A. polyethylene glycol (PEG)

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