Gene Transfer in Plants – Section 1

21. Opines that are present in crown gall tumor include

A. octopine
B. nopaline
C. agropine
D. all of these

Correct Answer: D. all of these

22. Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

A. pRK 2013
B. pRN 3
C. either (a) or (b)
D. none of these

Correct Answer: C. either (a) or (b)

23. Which of the following is true about T DNA?

A. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
B. Integration of T DNA occurs only at one specific site in the plant nuclear DNA
C. Integration of T DNA occurs at two specific sites in the plant nuclear DNA
D. Integration of T DNA occurs at one site that may be random in the plant nuclear DNA

Correct Answer: A. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA

24. Which of the following is not true about the direct repeats flanking T-DNA?

A. They are conserved between nopaline and octopine Ti-plasmids
B. These repeats are transferred intact to the plant genome
C. These are important in the integration mechanism
D. all of the above

Correct Answer: B. These repeats are transferred intact to the plant genome

25. The left segment of octopine T-DNA (TL) is necessary for

A. enzymes for agropine biosynthesis
B. tumor formation
C. conjugative transfer
D. all of these

Correct Answer: B. tumor formation

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