Immobilized Enzyme – Section 1


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11. For glucose isomerization by immobilized enzyme, the reactor generally used is

A. CSTR
B. plug flow
C. packed bed
D. fluidized bed

Correct Answer: C. packed bed
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12. If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient and a is the surface area of immobilized enzyme particles)

A. rp = ksa(CsbCs)
B. rp= ksaCsb
C. rp= ksaCs
D. rp= ksa(Csb+Cs)

Correct Answer: B. rp= ksaCsb
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13. The most commonly employed cross-linked polymer is the

A. polyacrylamide gel
B. collagen
C. celluloses
D. cation exchange resin

Correct Answer: A. polyacrylamide gel
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14. Which of the following is not the correct answer?

A.  Immobilized enzyme may show selectively altered chemical properties
B. Immobilized enzyme may show selectively altered physical properties
C. Immobilized enzyme may simulate the realistic natural environment where the enzyme comes from the cell
D. None of the above

Correct Answer: D. None of the above
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15. Commonly employed water-insoluble supports for the covalent attachment of enzyme include

A. acrylamide based polymers
B. polypeptides
C. dextran
D. all of these

Correct Answer: D. all of these
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