Immobilized Enzyme – Section 1

6. Which of the following is considered as a disadvantage to the adsorption method of immobilization?

A. It is possible to separate and purify the enzymes while being immobilized
B. The enzymes are not usually deactivated by adsorption
C. The adsorption is a reversible process
D. State of immobilization is very sensitive to solution pH, ionic strength and temperature

Correct Answer: D. State of immobilization is very sensitive to solution pH, ionic strength and temperature

7. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

A. equal to that of substrate consumption
B. more than that of substrate consumption
C. lesser than that of substrate consumption
D. is nothing to do with the substrate consumption

Correct Answer: A. equal to that of substrate consumption

8. The immobilized technique involving chemical method is

A. covalent bond formation dependent
B. non-covalent bond formation dependent
C. both (a) and (b)
D. ionic bond formation dependent

Correct Answer: A. covalent bond formation dependent

9. For a steady state condition, the change of substrate concentration (dCs/dt) is

A. zero
B. 1
C. >1
D. <1

Correct Answer: A. zero

10. Damkohler number(NDa) is

A. the ratio of the maximum reaction rate to the maximum mass transfer rate
B. the ratio of the minimum reaction rate to the maximum mass transfer rate
C. the ratio of the maximum reaction rate to the minimum mass transfer rate
D. the ratio of the minimum reaction rate to the minimum mass transfer rate

Correct Answer: A. the ratio of the maximum reaction rate to the maximum mass transfer rate

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