Protein Purification – Section 1

1. During successful purification scheme, this may be expected that the

A. specific activity increases
B. specific activity decreases
C. number of proteins in the sample decreases
D. both (a) and (c)

Correct Answer: D. both (a) and (c)

2. In ion-exchange chromatography

A. proteins are separated on the basis of their net charge
B. proteins are separated on the basis of their size
C. proteins are separated on the basis of their shape
D. either (b) or (c)

Correct Answer: A. proteins are separated on the basis of their net charge

3. Which of the following may be added to stabilize the protein after yeast cells disruption?

A. NaCl
B. Protease inhibitor
C. AMP
D. All of these

Correct Answer: D. All of these

4. Gel-filtration chromatography separates on the basis of

A. size and shape using porous beads packed in a column
B. size using porous beads packed in a column
C. shape using porous beads packed in a column
D. none of the above

Correct Answer: A. size and shape using porous beads packed in a column

5. Affinity chromatography deals with the

A. specific binding of a protein constituents for another molecule
B. protein – protein interaction
C. protein – carbohydrate interaction
D. none of the above

Correct Answer: A. specific binding of a protein constituents for another molecule

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