Protein Purification – Section 1

6. A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

A. 1,000 units/mg
B. 10,000 units/mg.
C. 100,000 units/mg
D. 1,000,000 units/mg

Correct Answer: C. 100,000 units/mg

7. Proteins separation can be carried out on the basis of

A. net charge
B. solubility in salt solutions
C. size or mass
D. all of these

Correct Answer: D. all of these

8. The best way to determine the location of protein in the purification scheme is to measure the

A. rate of ATP synthesis
B. changes in the refractive index
C. UV absorption
D. mass spectroscopy of the protein

Correct Answer: A. rate of ATP synthesis

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