Physical and Chemical Agents – Section 1

1. Which of the following articles can be sterilized in an autoclave?

A. Gloves
B. Culture media
C. Dressing material
D. All of these

Correct Answer: D. All of these

2. Which of the following disinfectants act by disrupting microbial membranes?

A. Cationic detergents
B. Halogens
C. Heavy metals
D. Aldehydes

Correct Answer: A. Cationic detergents

3. Which of the following is best to sterilize heat-labile solutions?

A. Dry heat
B. Autoclave
C. Membrane filtration
D. Pasteurization

Correct Answer: C. Membrane filtration

4. The time required to kill 90% of the microorganisms in a sample at a specific temperature is the

A. decimal reduction time
B. thermal death point
C. F value
D. D value

Correct Answer: D. D value

5. Which of the following is best used for long term storage of microbial samples when carried out properly?

A. Storage in a freezer at -10°C
B. Storage in a freezer at ultra-low temperatures (-70°C)
C. Storage in a refrigerator on an agar slant
D. Storage on a Petri plate at room temperature

Correct Answer: B. Storage in a freezer at ultra-low temperatures (-70°C)

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