Enzymes and Kinetics – Section 1

6. In competitive inhibition, a factor is obtained from the measurement of

A. Vmax
B. KM
C. Y-intercept in Lineweaver-Burk Plot
D. None of these

Correct Answer: B. KM
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7. Which of these proteases is not a cysteine active site protease?

A. Calpain
B. Cathepsin D
C. Papain
D. None of the above

Correct Answer: B. Cathepsin D
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8. Given an enzyme with a Km = 10m M and Vmax = 100 m mol/min. If [S] = 100 m M, which of the following will be true?

A. A 10 fold increase in Vmax would increase velocity 10 fold y
B. A 10 fold decrease in Km would increase velocity
C. Both (a) and (b)
D. A 10 fold increase in Vmax would decrease velocity 20 fold

Correct Answer: A. A 10 fold increase in Vmax would increase velocity 10 fold y
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9. The conformational change in an enzyme after the substrate is bound that allows the chemical reaction to proceed, can be explained by

A. induced fit
B. transition
C. fit and fine
D. Pasteur

Correct Answer: A. induced fit
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10. The active site of an enzyme remains

A. at the center of globular proteins
B. rigid and does not change shape
C. complementary to the rest of the molecule
D. none of the above

Correct Answer: D. None of the above
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