Protein Stability – Section 1

6. Which of the following is the most correct?

A. Charged amino acids are never buried in the interior of a protein
B. Charged amino acids are seldom buried in the interior of a protein
C. All hydrophobic amino acids are buried when a protein folds
D. Tyrosine is only found in the interior of proteins

Correct Answer: B. Charged amino acids are seldom buried in the interior of a protein

7. Which of the following forces is the most unfavorable for protein folding?

A. Conformational entropy
B. Hydrophobic interactions
C. Vander Waals interactions
D. Electrostatic interactions

Correct Answer: A. Conformational entropy

8. Since ΔG° = -RTlnK

A. a 10-fold increase in K decreases ΔG° by about 10-fold
B. a 10-fold decrease in K decreases ΔG° by about 2.3*RT
C. a 10-fold increase in K decreases ΔG° by about 2.3*RT
D. a 10-fold decrease in K increases ΔG° by about 10-fold

Correct Answer: C. a 10-fold increase in K decreases ΔG° by about 2.3*RT

9. The correlation between free energy ΔG transfer between the aqueous/organic phases and the surface area of amino acid residues

A. reflects the reduction in solvent-accessible area during protein folding
B. is only meaningful for the polar amino acids
C. ignores the important contribution of the peptide bond
D. is similar to effects seen with SDS denaturation

Correct Answer: A. reflects the reduction in solvent-accessible area during protein folding

10. If the egg white protein, ovalbumin, is denatured in a hard-boiled egg, then which of the following is least affected?

A. The primary structure of ovalbumin
B. The secondary structure of ovalbumin
C. The tertiary structure of ovalbumin
D. The quaternary structure of ovalbumin

Correct Answer: A. The primary structure of ovalbumin

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