Polymerase Chain Reaction – Section 1

1. Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?

A. HIV-1 and HIV-2 viruses
B. Hepatitis B virus
C. Mycobacterium tuberculosis
D. All of these

Correct Answer: D. All of these

2. Polymerase chain reaction basically consists of

A. two steps
B. three steps
C. four steps
D. five steps

Correct Answer: B. three steps

3. The PCR/sequencing approach to rRNA is most likely to give information about

A. the species to which the bacteria are most closely related
B. morphology of the bacterium
C. the type of energy metabolism the bacterium has
D. whether the bacterium is motile

Correct Answer: A. the species to which the bacteria are most closely related

4. The efficiency of amplification in PCR in later cycles is reduced due to

A. reduction in substrate concentration
B. insufficient enzyme and time to synthesize mass quantity of DNA
C. build up of PCR product which competes with primers for hybrid formation
D. all of these

Correct Answer: D. all of these

5. The original enzyme(s) used in PCR reaction is/are known as

A. E. coli DNA polymerase
B. E. coli RNA polymerase
C. Taq polymerase
D. all of these

Correct Answer: A. E. coli DNA polymerase

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