Free Online USMLE Step 1 Questions and Answers 8

7. A researcher develops 2 functional mRNA sequences composed of CUC and CUU trinucleotide repeats, respectively. He subsequently incubates these mRNAs in a solution containing functional ribosomes and tRNAs charged with the appropriate amino acids. After several hours, it is found that both mRNA sequences produce polypeptide chains containing leucine repeats. This observed finding is due to which of the following genetic principles?

A. Ambiguity
B. No punctuation
C. Transition
D. Universality
E. Wobble

Correct Answer: E

Answer Explanation:

(Choice A) The genetic code is not ambiguous as each codon is associated with only a single amino acid.
(Choice B) The genetic code is read sequentially from a starting point and has no internal punctuation as each codon is adjacent to the next without spacer nucleotides in between.
(Choice C) Genetic transition refers to a point mutation that results in replacement of a purine nucleotide for another purine or a pyrimidine nucleotide for another pyrimidine. In contrast, transversion refers to a point mutation that results in the replacement of a purine nucleotide for a pyrimidine or a pyrimidine nucleotide for a purine.
(Choice D) The genetic code is almost universal as amino acid codons are nearly identical across species; however, mitochondria and some bacteria and single-celled eukaryotes deviate from the standard genetic code.

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8. A pharmaceutical researcher develops a new drug that affects bacterial protein synthesis. In an experiment, Escherichia coli is exposed to the drug and serially cultured in media containing tagged nucleotides and amino acids. It is found that the drug inhibits molecules that recognize the highlighted codon in the bacterial mRNA fragment shown in the image below.

5′ — ACG CUA CCA UUG CAA GUU AGC UAA AUA GCG UUC — 3′

Which of the following molecules is the most likely target of this drug?

A. Charged tRNA
B. Elongation factor 2
C. Releasing factor 1
D. snRNP
E. Transcription factor II D
F. Uncharged tRNA

Correct Answer: C

Answer Explanation:

(Choice A) Charged tRNA delivers amino acids to the protein synthesis complex. The anticodon on a tRNA molecule recognizes the corresponding codon on mRNA, assuring proper amino acid sequencing.
(Choice B) Elongation factors facilitate tRNA binding and the translocation steps of protein synthesis.
(Choice D) Transcription produces a pre-mRNA molecule containing both introns and exons. Splicing is a posttranscriptional modification in which intrans are removed from pre-mRNA via small nuclear ribonucleoproteins (snRNPs).
(Choice E) Initiation of gene transcription is governed by the binding of transcription factors to the regulatory region of the gene. Transcription factor II D binds to the TATA promoter sequence located – 25 bases upstream from the coding region of the gene.
(Choice F) During protein synthesis, uncharged tRNA (lacking an amino acid) does not interact with mRNA and ribosomes.

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9. A pharmaceutical researcher performs preclinical testing on a novel chemotherapeutic drug. When rat embryos are exposed to this drug during an early stage of organogenesis, they develop severe skeletal malformations. Further genetic analysis reveals that the drug causes mutations in numerous homeobox genes containing highly conserved 180 base pair DNA sequences. The genes affected by this drug most likely code for which of the following proteins?

A. Cell surf ace receptors
B. Cytoplasmic enzymes
C. DNA replication enzymes
D. Structural proteins
E. Transcription regulators
F. Transport proteins

Correct Answer: E

Answer Explanation:

(Choices A, B, C, 0, and F) Homeobox genes do not typically encode cell surface receptors, cytoplasmic enzymes, DNA replication enzymes, structural proteins, or transport proteins.

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