Cell Cultures and Characteristics – Section 1

1. Which of the following bacteria can grow in acidic pH?

A. Vibrio cholerae
B. Lactobacilli
C. Shigella
D. Salmonella

Correct Answer: B. Lactobacilli

2. Cell size and cell number can be measured using

A. hemocytometer
B. coulter counter
C. petroff-hausser counting chamber
D. none of these

Correct Answer: B. coulter counter

3. Which of the following is used for the proper maintenance and preservation of pure cultures?

A. Periodic transfer to fresh media
B. Preservation by overlaying cultures with mineral oil
C. Preservation by lyophilization
D. All of the above

Correct Answer: D. All of the above

4. In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

A. -60 to -78°C
B. -20 to -30°C
C. -30 to -48°C
D. -48 to -58°C

Correct Answer: A. -60 to -78°C

5. The solidifying agent commonly used in preparation of media is/are

A. agar
B. silica gel
C. both (a) and (b)
D. none of these

Correct Answer: C. both (a) and (b)

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