Special Proteins and Crop Improvement – Section 1

6. Which of the following have been used for cryo-preservation of germplasm with 90% tissue survival and 10%plant regeneration that are used in the international exchange of germplasm?

A. Meristem tip cultures
B. Embryo cultures
C. Both (a) and (b)
D. Embryo cultures supplemented with GA3 and coconut milk

Correct Answer: A. Meristem tip cultures

7. Establishment of embryogenic cell suspensions with high regeneration potential is a pre-requisite for successful

A. protoplast culture
B. detection of nucleic acid sequence
C. restriction of fragment length polymorphisms
D. microinjection

Correct Answer: A. Meristem tip cultures

8. Which of the following technique can be used to develop nematode-resistant plants?

A. Embryo-rescue
B. Mutation
C. Non-recombinant DNA
D. All of these

Correct Answer: D. All of these

9. Random amplified polymorphic DNA (RAPD) is a method that

A. stimulate the production of sense RNA to compensate
B. activate the expression of all genes in a biochemical pathway
C. eliminate the expression of all genes in a biochemical pathway
D. reveals intra-specific variation and diversity between species

Correct Answer: D. reveals intra-specific variation and diversity between species

10. Which of the following transfers a set of genes encoded in a region called T-DNA of the Ti-plasmid into cells at wound positions?

A. A. tumefaciens
B. L. peruvianum
C. L. hirsutum
D. A. sterilis

Correct Answer: A. A. tumefaciens

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