FT IR Spectroscopy – Section 1

11. How do you turn a signal recorded in the time domain into a frequency domain signal?

A. Fourier transformation
B. Measurement of peak areas
C. By use of a Michelson interferometer
D. None of the above

Correct Answer: A. Fourier transformation

12. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?

A. 2
B. 4
C. 3
D. 6

Correct Answer: A. 2

13. In immunofluroscence microscopy, fluroscent compounds are attached to

A. an antibody specific for the subcellular structure
B. an antigen specific for the subcellular structure
C. bound antibody specific for the subcellular structure
D. none of these

Correct Answer: A. an antibody specific for the subcellular structure

14. An FT-IR instrument record a signal in the

A. time domain
B. frequency domain
C. both (a) and (b)
D. none of these

Correct Answer: A. time domain

15. When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens?

A. The processional frequency of the nucleus increases
B. The nucleus spins faster
C. The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field
D. none of the above

Correct Answer: C. The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field

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