Fermentation Reactors – Section 2

26. Saccharomyces cerevisiae when grown in a high glucose concentration medium, the cells activity depends on which of the following?

A. High concentration of dissolved oxygen
B. Low concentration of dissolved oxygen
C. No effect of dissolved oxygen
D. High concentration of dissolved oxygen and sodium salts

Correct Answer: C. No effect of dissolved oxygen

27. Continuous culture has an advantage over to a batch culture as

A. continuous reactors do not need to be shut down and cleaned as regularly as a batch reactor
B. most downstream and upstream processes are continuous in nature and thus the operation of a continuous bioreactor can be better integrated into the overall manufacturing process
C. cells can be maintained at a constant physiological state simply by setting the dilution rate
D. all of the above

Correct Answer: D. all of the above

28. In the batch-fed mode, the substrate will be added

A. only at the beginning of the fermentation process
B. periodically throughout the fermentation process
C. only at the end of the fermentation process
D. substrate is never added

Correct Answer: C. only at the end of the fermentation process

29. When Escherichia coli grows in a well-aerated medium, which of the following will occur?

A. Fructose di-phosphate accumulation inside the cells leading to cell respiration
B. Fructose di-phosphate accumulation inside the cells leading to fermentation
C. Fructose di-phosphate will accumulate regardless of the medium composition
D. Fructose di-phosphate will accumulate inside the cells leading to anaerobic respiration

Correct Answer: B. Fructose di-phosphate accumulation inside the cells leading to fermentation

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