Animal Cell Culture and Regulation – Section 1

26. The technique of organ culture may be divided on the basis of employing

A. solid medium
B. liquid medium
C. both (a) and (b)
D. semi-solid medium

Correct Answer: C. both (a) and (b)

27. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

A. oxidative pathway
B. anaerobic pathway
C. both (a) and (b)
D. none of these

Correct Answer: A. oxidative pathway

28. An established cell line can be called where it has been subcultured at least

A. 70 times at an interval of 3 days between subcultures
B. 40 times at an interval of 3 days between subcultures
C. 70 times at an interval of 1 day between subcultures
D. 50 times at an interval of 3 days between subcultures

Correct Answer: A. 70 times at an interval of 3 days between subcultures

29. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

A. 0.05-5 nmol of O2/cell/h
B. 5 – 10 nmol of O2/cell/h
C. 0.05-5 pmol of O2/cell/h
D. 5 – 10 pmol of O2/cell/h

Correct Answer: C. 0.05-5 pmol of O2/cell/h

30. Which of the following is not a source of energy in active muscle cells?

A. Creatine phosphate
B. ATP
C. Lactic acid
D. Glucose

Correct Answer: C. Lactic acid

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