Animal Cell Culture and Regulation – Section 1

11. What is the concentration of CO2 required for culturing animal cells?

A. 2-5%
B. 1-10%
C. 10-15%
D. 15-20%

Correct Answer: B. 1-10%

12. Which of the following is correct?

A. ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
B. ECL and PCL can be established in suspension cultures
C. PCL can be established in suspension cultures
D. none of the above

Correct Answer: A. ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)

13. Which of the following are the metabolic products of glucose and glutamine?

A. CO2 and NH3
B. CO2 and lactate
C. Lactate and ammonium
D. Lactate only

Correct Answer: C. Lactate and ammonium

14. To prevent the accumulation of lactate

A. low glutamine concentration is required
B. high glutamine concentration is required
C. low glucose concentration is required
D. high glucose concentration is required

Correct Answer: C. low glucose concentration is required

15. What are the main constituents of culture for animal cell growth?

A. Glucose and Glutamine
B. Growth factors
C. Cytokines
D. All of these

Correct Answer: A. Glucose and Glutamine

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